Analysis Note

ControlRat Brain

Application

Research CategoryNeuroscience

Immunohistochemistry:
Dilutions of 1:10 – 1:500 were tested on rat brain lysate.
Can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Suggested concentrations start at 1-3 μg/mL.

Western blot analysis can be done at final concentrations of 1-3 μg/mL.

Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurotransmitters & Receptors

This Anti-Glutamate Receptor 4 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glutamate receptor 4 (UniProt: P48058; also known as GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic AMPA 4, GluA4) is encoded by the GRIA4 (also known as GLUR4) gene (Gene ID: 2893) in human. The postsynaptic actions of glutamate are mediated by a variety of receptors that are named according to their selective agonists. GluR-4 is a multi-pass membrane protein that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. It displays greater affinity for AMPA. GluR-4 is synthesized with a signal peptide (aa 1-20), which is cleaved off in the mature form. GluR-4 can exist as a homotetramer or heterotetramer of pore-forming glutamate receptor subunits, tetramers can also form by the dimerization of dimers. GluR-4 is generally palmitoylated and becomes de-palmitoylated upon glutamate stimulation. Palmitoylation on Cys 611 leads to its Golgi retention and reduced cell surface expression. However, palmitoylation at Cys 837 does not affect cell surface expression, but can regulate stimulation-dependent endocytosis. GluR-4 can undergo phosphorylation at Serine 862 by PKC gamma and this phosphorylation increases plasma membrane-associated GRI4 expression.

Immunogen

Epitope: C-Terminus

Synthetic linear peptide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 06-308

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in mouse and rat brain membrane.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Glutamate Receptor 4 in mouse and rat brain membrane.

Specificity

The immunogen sequence shares 100% conservation with chicken, mouse, rat, oppossum, human, chimp, and monkey.

Cat. # AB1508 recognizes C-Terminus of GluR4.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.

Target description

Approx. 100 kDa